Objective: To analyze the effect of comprehensive nursing on the motor function and pain level of patients with ankylosing spondylitis. Methods: The total number of patients involved in the study was 150, all of whom were diagnosed with ankylosing spondylitis from January 2023 to December 2023. After random number table grouping, the number of patients in each group was 75. The control group received routine care, while the observation group received comprehensive care. By comparing and observing relevant indicators, clarify the application effects of different nursing methods. Results: The total effective rate of nursing in the observation group was higher than that in the control group, and the satisfaction was higher than that in the control group, with statistical significance (P<0.05). There was no statistically significant difference (P>0.05) in the comparison of the six BASDAI scoring questions before intervention. After intervention, the evaluation of the observation group was lower than that of the control group (P<0.05). Conclusion: With the support of comprehensive nursing, the motor function and pain level of patients with ankylosing spondylitis have significantly improved, the nursing effect is significant, and the patient satisfaction is high.